Mouse IL-4 ELISA Kit

ArtNr	ELM-IL4-5
Hersteller	Raybiotech
Menge	5 Plate Kit
Quantity options	1 Plate Kit 2 Plate Kit 5 Plate Kit 1 Plate Kit 2 Plate Kit 5 Plate Kit
Kategorie	Oncology Cell Biology Cell Culture Cytokines ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Lung Cancer Bladder Cancer
Typ	Elisa-Kit
Specific against	Mouse (Murine, Mus musculus)
Host	Hamster - Armenian
Sensitivity	0.6 pg/ml
Citations	1. Gu X., Zie Y., Wang S., Peng X., Lai S., Yang G. Immune response induced by candidate Sarcoptes scabiei var. cuniculi DNA vaccine encoding paramyosin in mice. Exp Appl Acarol. 2014 Apr 13. [Epub ahead of print] 2. Jian Y., Zou Y., Chen S., Zhu C., Wu A., Liu Y., Ma L., et al. The anti-inflammatory effect of donepezil on experimental autoimmune encephalomyelitis in C57 BL/6 mice. Neuropharmacology 73 (2013) 415-424 3. Tian X., et al. In Vivo Immunotoxicity of SiO2(Y0.5Gd0.45Eu0.05)2O3 as Dual-Modality Nanoprobes. Int. J. Mol. Sci. 2014, 15, 13649-13662, doi:10.3390/ijms150813649 4. Ahmad SF., et al. Naringin Attenuates the Development of Carrageenan-Induced Acute Lung Inflammation Through Inhibition of NF-kappab, STAT3 and Pro-Inflammatory Mediators and Enhancement of IkappaBalpha and Anti-Inflammatory Cytokines. Inflammation, 2014 Online first view August, DOI: 10.1007/s10753-014-9994-y 5. Tian X., et al. Toxicity evaluation of Gd2O3SiO2 nanoparticles prepared by laser ablation in liquid as MRI contrast agents in vivo. International Journal of Nanomedicine. 2014 Aug 21,9:4043-53. doi: 10.2147/IJN.S66164. 6. Matsushita A., et al. Close relationship between T helper (Th)17 and Th2 response in murine allergic contact dermatitis. Clinical and Experimental Dermatology, epub ahead of print. 5 SEP 2014. DOI: 10.1111/ced.12425 7. Kawahara, K. Cooperative Therapeutic Action of Retinoic Acid Receptor and Retinoid X Receptor Agonists in a Mouse Model of Alzheimer's Disease. Journal of Alzheimer's Disease 2014. Epub Ahead of Print. DOI: 10.3233/JAD-132720 8. Liu J., et al. Sub-10 nm Monoclinic Gd2O3:Eu3+ Nanoparticles as Dual-Modal Nanoprobes for Magnetic Resonance and Fluorescence Imaging. Langmuir, Article ASAP, 2014. DOI: 10.1021/la503228v
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Similar products	IL-4
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Category	ELISA Kits\|Sandwich ELISA
Manufacturer - Targets	IL-4
Shipping Temperature	Blue ice
Storage Conditions	≤-20°C
Description	RayBio® Mouse IL-4 ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples. Lead time: Typically ships within 1-2 business days. No Friday shipments.
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Design Principle	Sandwich-based
Method of Detection	Colorimetric
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Gene Symbols	IL4, IL-4
Kit Components	Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Other Materials Required	Distilled or deionized water Precision pipettes to deliver 2 ul to 1 ul volumes Adjustable 1-25 ul pipettes for reagent preparation 100 ul and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline	Prepare all reagents, samples and standards as instructed in the manual. Add 100 ul of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4C. Add 100 ul of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 ul of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 ul of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 ul of Stop Solution to each well. Read at 450 nm immediately.
Product Features	Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
UNSPSC Code	41116158
Result Output	Quantitative
Protein Name & Synonyms	Interleukin-4 (IL-4) (B-cell IgG differentiation factor) (B-cell growth factor 1) (B-cell stimulatory factor 1) (BSF-1) (IGG1 induction factor) (Lymphocyte stimulatory factor 1)
Manufacturer - Specificity	This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Recommended Dilution	2 fold

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ELM-IL4-5.pdf

ELM-IL4-5-datasheet.pdf

ELM-IL4-5-safety-datasheet.pdf