Rat IL-6 ELISA Kit

ArtNr	ELR-IL6-5
Hersteller	Raybiotech
Menge	5 Plate Kit
Quantity options	1 Plate Kit 2 Plate Kit 5 Plate Kit 1 Plate Kit 2 Plate Kit 5 Plate Kit
Kategorie	Oncology Metabolism Diabetes Glucose Homeostasis By Cell Type Carcinoma Hematology Leukemia Cell Biology Obesity Cell Culture Cytokines Cell Status Necrosis ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Lung Cancer
Typ	Elisa-Kit
Specific against	Rat (Rattus norvegicus)
Host	Hamster - Armenian
Sensitivity	30 pg/ml
Citations	1. Tang W, Chen Z, Wu W, et al. Renal protective effects of early continuous venovenous hemofiltration in rhabdomyolysis: improved renal mitochondrial dysfunction and inhibited apoptosis. Artif Organs. 2013 Apr,37(4):390–400. 2. Gül FC, Turgut B, Dagli F, et al. The comparison of the impact of ghrelin and tacrolimus on vitreous cytokine levels in an experimental uveitis model. Graefes Arch Clin Exp Ophthalmol. 2013 Apr,251(4):1235–1241. 3. Brito LCW, Dalbo S, Striechen TM, et al. Experimental periodontitis promotes transient vascular inflammation and endothelial dysfunction. Arch Oral Biol. 2013,58(9):1187–1198. 4. Albayrak A, Halici Z, Polat B, et al. Protective effects of lithium: A new look at an old drug with potential antioxidative and anti-inflammatory effects in an animal model of sepsis. Int Immunopharmacol. 2013 May,16(1):35–40. 5. Cadirci E, Halici Z, Bayir Y, et al. Peripheral 5-HT7 receptors as a new target for prevention of lung injury and mortality in septic rats. Immunobiology. 2013. 6. Zhou C, He X, Xue D, Xu N. Elevated expression of hepcidin post-renal ischemia reperfusion injury. Acta Biochim Biophys Sin (Shanghai). 2013 Apr,45(4):342–344. 7. Zhou C, He X, Xue D, Xu N. Elevated expression of hepcidin post-renal ischemia reperfusion injury. Acta Biochim Biophys Sin (Shanghai). 2013 Apr,45(4):342–344. 8. Gullaiya S, Nagar A, Dubey V, et al. Modulation of disease related immune events by demethoxycurcumin against autoimmune arthritis in rats. Biomed Aging Pathol. 2013 Jan,3(1):7–13. 9. Karakus E, Halici Z, Albayrak A, et al. Agomelatine: An antidepressant with new potent hepatoprotective effects on paracetamol-induced liver damage in rats. Hum Exp Toxicol. 2013 Aug,32(8):846–857. 10. Oyewo EB, Adewale A, Abdulfatai Ayoade A, Akanji MA. Repeated Oral Administration of Aqueous Leaf Extract of Moringa oleifera modulated immunoactivities in Wistar Rats. J Nat Sci Res. 2013,3(6):100–109. 11. Polat B, Cadirci E, Halici Z, et al. The protective effect of amiodarone in lung tissue of cecal ligation and puncture-induced septic rats: a perspective from inflammatory cytokine release and oxidative stress. Naunyn Schmiedebergs Arch Pharmacol. 2013 Jul,386(7):635–643. 12. Zhang Y., Zhang S., Cui N. Intravenous infusion of mesenteric lymph from severe intraperitoneal infection rats causes lung injury in healthy rats. World J Gastroenterol 2014 April 28, 20(16): 4771-4777 13. Chhabra G.S., Kulkarni K.S. Evaluation of anti-inflammatory activity of Vitex agnus castus leaves. Quantitative analysis of flavonoids as possible active constituents. ca. 183 ca. Journal of Pharmacognosy and Phytochemistry 2014, 3 (1): 183-189 14. Sun Peng., Zhou K., Wang S., Li P., Chen S., et al. Involvement of MAPK/NF-kappaB Signaling in the Activation of the Cholinergic Anti-Inflammatory Pathway in Experimental Colitis by Chronic Vagus Nerve Stimulation. PLOS One Published: August 02, 2013 DOI: 10.1371/journal.pone.0069424 15. Aydin A., et al. Treatment with alpha-lipoic acid enhances the bone healing after femoral fracture model of rats. Naunyn-Schmiedeberg's Archives of Pharmacology 2014 DOI 10.1007/s00210-014-1021-1
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Similar products	IL-6
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Category	ELISA Kits\|Sandwich ELISA
Manufacturer - Targets	IL-6
Shipping Temperature	Blue ice
Storage Conditions	≤-20°C
Description	RayBio® Rat IL-6 ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples. Lead time: Typically ships within 1-2 business days. No Friday shipments.
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Design Principle	Sandwich-based
Method of Detection	Colorimetric
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Gene Symbols	IL6, IL-6
Kit Components	Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Other Materials Required	Distilled or deionized water Precision pipettes to deliver 2 ul to 1 ul volumes Adjustable 1-25 ul pipettes for reagent preparation 100 ul and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline	Prepare all reagents, samples and standards as instructed in the manual. Add 100 ul of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4C. Add 100 ul of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 ul of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 ul of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 ul of Stop Solution to each well. Read at 450 nm immediately.
Product Features	Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
UNSPSC Code	41116158
Result Output	Quantitative
Protein Name & Synonyms	Interleukin-6 (IL-6)
Manufacturer - Specificity	This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1 alpha, IL-1 beta, IL-4, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3 alpha, beta-NGF, TIMP-1, TNF-alpha.
Recommended Dilution	2 fold

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