Recombinant Human TNF ALPHA

Hersteller Raybiotech
Kategorie
Typ Proteins
Specific against Human
Menge 50 ug
ArtNr 229-20151
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Description
229-20151 is an E. coli-derived recombinant protein corresponding to the C-terminal 157 amino acids of human Tumour Necrosis Factor Alpha (TNFa), a cytokine which exerts cytotoxic effects on a wide range of tunour cells.

229-20151 has an ED50 of <0.05 ng/ml as determined by an assay measuring cytolysis of L929 cells in the presence of Actinomycin D.
Protein Name
TNF ALPHA
Molecular Weight (kDa)
17.4kD (157 Amino acid sequence)
Purity
>98% by SDS PAGE and HPLC analysis
Concentration
Total protein concentration ca.0.1 mg/ml after reconstitution
Preservative
None present
Endotoxin Level
<0.1ng/ug
Buffers
TRIS buffered saline
Protein Type
Recombinant Protein
Recommended Applications
ELISA (0.2-0.4ng/well), Functional Assays (0.05-20.0ng/ml), Western Blotting (1.5-3.0ng/lane)
Reconstitution
Reconstitute with 0.5 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommends that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Storage/stability
Prior to reconstitution store at +4° C. Following reconstitution store at -20° C.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. This protein may be stored for3 months from date of reconstitution

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Menge: 50 ug
Lieferbar: In stock
Listenpreis: 528,00 €
Preis: 528,00 €
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