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Recombinant Human TNF ALPHA

Recombinant Human TNF ALPHA

Manufacturer	Raybiotech
Category	Peptides & Proteins Recombinant Proteins
Type	Proteins
Specific against	Human
Amount	50 ug
Item no.	229-20151
eClass 6.1	34160400
eClass 9.0	42020190
Available
Description	229-20151 is an E. coli-derived recombinant protein corresponding to the C-terminal 157 amino acids of human Tumour Necrosis Factor Alpha (TNFa), a cytokine which exerts cytotoxic effects on a wide range of tunour cells. 229-20151 has an ED₅₀ of <0.05 ng/ml as determined by an assay measuring cytolysis of L929 cells in the presence of Actinomycin D.
Protein Name	TNF ALPHA
Molecular Weight (kDa)	17.4kD (157 Amino acid sequence)
Purity	>98% by SDS PAGE and HPLC analysis
Concentration	Total protein concentration ca.0.1 mg/ml after reconstitution
Preservative	None present
Endotoxin Level	<0.1ng/ug
Buffers	TRIS buffered saline
Protein Type	Recombinant Protein
Recommended Applications	ELISA (0.2-0.4ng/well), Functional Assays (0.05-20.0ng/ml), Western Blotting (1.5-3.0ng/lane)
Reconstitution	Reconstitute with 0.5 ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommends that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. N.B. For functional studies do not add sodium azide
Storage/stability	Prior to reconstitution store at +4° C. Following reconstitution store at -20° C. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. This protein may be stored for3 months from date of reconstitution

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.