Recombinant Human HE4 Protein, HEK293

Proteins|Recombinant Proteins|Human Proteins

Ambient temperature

The predicted size of HE4 protein is 12 kDa. The affinity purified recombinant human HE4 showed two bands: the major band is about 20 kDa, and the minor band is about 12 kDa. Both bands were confirmed by Western blotting using anti-HE4 antibody. The 20-kDa band may represent N-glycosylated form (Ronny Drapkin, et al. Cancer Res 2005, 65(6): 2162-9).

SDS-PAGE under reducing conditions and visualized by Coomassie blue staining

12352202

Glu31-Phe124

Lyophilized powder

Determined by BCA protein assay kit (Thermo Scientific)

Not determined

Figure 1. Deglycosylation of purified recombinant proteins. Purified proteins were untreated (Lane 2) or treated with protein deglycosylation enzymes under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one major band at the expected size, thus indicating that the untreated recombinant protein (Lane 2) was glycosylated.Lane 1: Protein standard ladder (kDa).Lane 2: Untreated protein under reducing conditions.Lane 3: Treated protein with deglycosylation enzymes under native conditions.Lane 4: Treated protein with deglycosylation enzymes under reducing conditions.Lane 5: Deglycosylation mixture only without target proteins.