Mebiol Gel

Note:
This product is packaged with RP Drying Agent packs to absorb moisture, oxygen and corrosive gases. It is therefore subject to European Commerce Area Import Regulation (EC) No. 1272/2008 - Classification, Labeling, and Packaging of Substances and Mixtures (CLP). Applies to EEA (i.e. EU27 countries, Iceland, Liechtenstein, Norway, UK).
For additional information, see the following:
OSHA Europe
RP Agent Manufactuer' s StatementIntroduction to Mebiol® GelHydrogels are a diverse class of polymeric materials characterized by their network-like structure and high water content. Hydrogels of many kinds have found a wide variety of applications in medicine and life science research weighted towards, but not at all limited to three-dimensional cell culture, tissue engineering, and drug delivery. Properties highly favorable to cell culture and tissue engineering applications prompted the commercialization of Mebiol® Gel, a copolymer of poly(N-isopropylacrylamide) and poly(ethylene glycol) (PNIPAAm-PEG) for research purposes in the early 2000' s.
Mebiol® Gel' s defining feature, in contrast to other commercially available hydrogels, is its temperature reversible sol-gel transition. When cooled, Mebiol® Gel is a sol (handles like a liquid) but becomes a rigid hydrogel at higher temperatures. In practice, this means extremely easy cell handling. Cultures are seeded into cooled Mebiol® Gel and recovered conveniently by cooling the culture vessel and centrifugation. In the gel state, the highly lipophylic environment of the Mebiol® Gel presents an efficient niche for cell proliferation, cell communication, gas and mass exchange, and protection of cells and tissue from shear forces.
Features· Easy handling
· Non-toxic, biocompatible
· 100% synthetic, pathogen free
· High transparency for cell observation
· Proven performance.PreparationApplication· Stem cell and pluripotent stem cell culture, expansion, and differentiation
· Spheroid culture
· Cell implantation
· Organ and Tissue Regeneration
· Drug Delivery
· Non-cell culture application
Application ExamplesThe strong positive alkaline phosphatase staining of Macaca (primate) ES cells cultured in Mebiol® Gel suggests undifferentiation.Left: 2D on Feeder Cells
Right: 3D Culture in Mebiol Gel
(without LF, day 5) 3) Selective separate culture of somatic stem cell (mouse embryo skin origin)Isolation of Epithelial Stem Cells from Dermis by a Three-dimensional Culture System
Journal of Cellular Biochemistry, 98 (1), 174-184 (2006) PMID: 16408300 4) Cartilage tissue reproduction by in vitro 3D culture of cartilage cellChondrocytes Containing Growth Factors in a Novel Thermoreversible Gelation Polymer Scaffold
Tissue Engineering, 12 (5), 1237-1245 (2006) 5) Bone induction by in vitro 3D culture of human mesenchymal cell stem cell (hMSC)Gene expression profile of human mesenchymal stem cells during osteogenesis in three-dimensional thermoreversible gelation polymer
Biochem. Biophys. Res. Commun., 317, 1103-1107 (2004). PMID: 15094382 6) Production of hepatitis C viruses (HCV) by 3-D culture of human hepatocyte cell lineProduction of infectious hepatitis C virus particles in three-dimensional cultures of the cell line carrying the genome-length dicistronic viral RNA of genotype 1b
Virology, 351 (2), 381-392 (2006) PMID: 16678876 7) Passage control by local heating (on chip cell sorter system)On-Chip Cell Sorting System Using Laser-Induced Heating of a Thermoreversible Gelation Polymer to Control Flow, Y. Shirasaki, J. Tanaka, H. Makazu, K. Tashiro, S. Shoji, S. Tsukita, T. Funatsu,
Anal. Chem., 78, 695-701 (2006) PMID: 16448041moreTechnical Notes Mebiol® Gel Literature Citations and References
Mebiol® Gel Frequently Asked Questions (FAQ)Dynamic Viscoelastic Property of Mebiol® Gel
Temperature dependence of the dynamic moduli of the aqueous solution of Mebiol® Gel at a concentration of 10 wt% in distilled water. Storage modulus (G' , solid lines) and loss modulus (G' ' , broken lines) were measured on heating (closed symbols) and cooling (open symbols) at the oscillatory frequency of 1 Hz.