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Recombinant Human E-Cadherin Protein (ECD, His Tag) Europäischer Partner

ArtNr 10204-H08H-200
Hersteller Sino Biological
Menge 200 ug
Quantity options 1 mg 100 ug 2 mg 200 ug 4 mg
Kategorie
Typ Proteins
Specific against Human (Homo sapiens)
Host Human
Konjugat/Tag HIS
Purity > 95 % as determined by SDS-PAGE
Sequence Met1-Ile707
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias Arc-1,CD324,CDHE,E-cad,E-Cadherin,ECAD,LCAM,UVO
Similar products CDH1
Lieferbar
Manufacturer - Conjugate / Tag
C-His
Shipping Temperature
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Storage Conditions
Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Antigen molecule name
CDH1
Protein Construction
A DNA sequence encoding the extracellular domain of human E-Cad precusor (NP_004351.1) (Met 1-Ile 707) was fused with a C-terminal polyhistidine tag.
Expressed Host
Human Cells
Bio-Activity
Measured by the ability of the immobilized protein to support the adhesion of MCF-7 human breast adenocarcinoma cells.
When cells are added to E-Cad coated plates (5 ug/mL, 100 uL/well), approximately 20%-40% of cells will adhere specifically after 90 minutes at 37 C.
Endotoxin
< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Gln 23 & Asp 155
Molecule Mass
The recombinant pro form of human E-Cad consists of 696 amino acids and has a calculated molecular mass of 77 kDa. As a result of glycosylation, the apparent molecular mass of rh E-Cad is approximately 80&95 kDa corresponding to the mature form and pro form respectively in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 200 ug
Lieferbar: In stock
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