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Human Endoglin/CD105 Matched ELISA Antibody Pair Set

Human Endoglin/CD105 Matched ELISA Antibody Pair Set

ArtNr	SEK10149-15
Hersteller	Sino Biological
Menge	15 Plates
Quantity options	15 Plates 5 Plates
Kategorie	Oncology Neuroscience Neuroinflammation Molecular Targets for Neuroscience Neural Development Antibodies By Cell Type Carcinoma ELISA Immunoassay Immunostaining Antibody Pairs
Typ	ELISA Pair Set
Applikationen	ELISA
Specific against	Human (Homo sapiens)
Konjugat/Tag	HRP
Sensitivity	The minimum detectable dose of human Endoglin (CD105 / ENG) was determined to be approximately 31.25 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	SEK10149
Similar products	ENG
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Manufacturer - Type	ELISA
Storage Conditions	Capture Antibody: Aliquot and store at -20C to -80C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20C to -80C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20C to -80C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80C for up to 1 month. Avoid repeated freeze-thaw cycles.
Antigen molecule name	ENG
Capture Ab	0.5 mg/mL of mouse anti-CD105 monoclonal antibody. Dilute to a working concentration of 2.0 ug/mL in CBS before coating
Detection Ab	0.5 mg/mL mouse anti-CD105 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1.0 ug/mL in detection antibody dilution buffer before use
Standard	Each vial contains 120 ng of recombinant CD105. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20C to -80C in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 1000 pg/mL is recommended
Principle of the test	The human Endoglin (CD105 / ENG) ELISA Pair Set is for the quantitative determination of human Endoglin. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Endoglin ( CD105 / ENG) coated on a 96-well plate. Standards and samples are added to thewells, and any Endoglin / CD105 present binds to the immobilized antibody. Thewells are washed and a horseradish peroxidase conjugated mouse anti-Endoglin /CD105 monoclonal antibody is then added, producing an antibody-antigen-antibody"sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Endoglin / CD105 present inthe sample. To end the enzyme reaction, the stop solution is added andabsorbances of the microwell are read at 450 nm.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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