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Human ICAM-3/CD50 Matched ELISA Antibody Pair Set

Human ICAM-3/CD50 Matched ELISA Antibody Pair Set

ArtNr	SEK10333-15
Hersteller	Sino Biological
Menge	15 Plates
Quantity options	15 Plates 5 Plates
Kategorie	Antibodies ELISA Immunoassay Immunostaining Antibody Pairs
Typ	ELISA Pair Set
Applikationen	ELISA
Specific against	Human (Homo sapiens)
Konjugat/Tag	HRP
Sensitivity	The minimum detectable dose of human ICAM3 was determined to be approximately 62.5 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	SEK10333
Similar products	ICAM3
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Manufacturer - Type	ELISA
Storage Conditions	Capture Antibody: Aliquot and store at -20C to -80C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20C to -80C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20C to -80C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80C for up to 1 month. Avoid repeated freeze-thaw cycles.
Antigen molecule name	ICAM3
Capture Ab	0.5 mg/mL of mouse anti-ICAM3 monoclonal antibody. Dilute to a working concentration of 2 ug/mL in CBS before coating.
Detection Ab	0.5 mg/mL mouse anti-ICAM3 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1 ug/mL in detection antibody dilution buffer before use
Standard	Each vial contains 40 ng of recombinant ICAM3. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20C to -80C in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 4 ng/mL is recommended.
Principle of the test	The human ICAM3 ELISA Pair Set is for the quantitative determination of human ICAM3. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for ICAM3coated on a 96-well plate. Standards and samples are added to the wells, andany ICAM3 present binds to the immobilized antibody. The wells are washed and ahorseradish peroxidase conjugated mouse anti-ICAM3 monoclonal antibody is thenadded, producing an antibody-antigen-antibody "sandwich". The wells are againwashed and TMB substrate solution is loaded, which produces color in proportionto the amount of ICAM3 present in the sample. To end the enzyme reaction, thestop solution is added and absorbances of the microwell are read at 450 nm.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 15 Plates

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