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Human PDGF alpha receptor/PDGFRA Matched ELISA Antibody Pair Set

Human PDGF alpha receptor/PDGFRA Matched ELISA Antibody Pair Set

ArtNr	SEK10556-15
Hersteller	Sino Biological
Menge	15 Plates
Quantity options	15 Plates 5 Plates
Kategorie	Oncology Neuroscience Neural Lineage Markers Neuroinflammation Antibodies By Cell Type Blastoma Carcinoma Glioma Hematology Leukemia Lymphoma Myeloma Melanoma Sarcoma ELISA Immunoassay Immunostaining Antibody Pairs
Typ	ELISA Pair Set
Applikationen	ELISA
Specific against	Human (Homo sapiens)
Konjugat/Tag	HRP
Sensitivity	The minimum detectable dose of Human PDGFRA / CD140a was determined to be approximately 31.25 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	SEK10556
Similar products	PDGFRA
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Manufacturer - Type	ELISA
Storage Conditions	Capture Antibody: Aliquot and store at -20C to -80C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20C to -80C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20C to -80C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80C for up to 1 month. Avoid repeated freeze-thaw cycles.
Antigen molecule name	PDGFRA
Capture Ab	0.5 mg/mL of mouse anti-PDGFRA monoclonal antibody. Dilute to a working concentration of 2 ug/mL in CBS before coating.
Detection Ab	0.4 mg/mL rabbit anti-PDGFRA polyclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5 ug/mL in detection antibody dilution buffer before use.
Standard	Each vial contains 35 ng of recombinant PDGFRA. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20C to -80C in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 2 ng/mL is recommended.
Principle of the test	The human PDGFRA / CD140a ELISA Pair Set is for the quantitative determination of human PDGFRA / CD140a. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for PDGFRA / CD140a coated on a 96-well plate. Standards and samples are added to the wells, and any PDGFRA / CD140a present binds to the immobilized antibody. The wells are washed and a biotinylated rabbit anti-PDGFRA / CD140a polyclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". To produces color in proportion to the amount of PDGFRA / CD140a present in the sample strepavidin-HRP and TMB substrate solution are loaded. The absorbances of the microwell are read at 450 nm.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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