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Human CD48 / SLAMF2 / BCM1 ELISA Pair Set

Human CD48 / SLAMF2 / BCM1 ELISA Pair Set

ArtNr	SEK10797-5
Hersteller	Sino Biological
Menge	5 Plates
Quantity options	15 Plates 5 Plates
Kategorie	Neuroscience Aging & Neurological Disorders Antibodies ELISA Immunoassay Immunostaining Antibody Pairs
Typ	ELISA Pair Set
Applikationen	ELISA
Specific against	Human (Homo sapiens)
Konjugat/Tag	Biotin
Sensitivity	The minimum detectable dose of human CD48 (BCM-1 / SLAMF2) was determined to be approximately 15.6 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	SEK10797
Similar products	CD48
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Storage Conditions	Capture Antibody: Aliquot and store at -20C to -80C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Aliquot and store at -20C to -80C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20C to -80C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80C for up to 1 month. Avoid repeated freeze-thaw cycles. Streptavidin-HRP: Store at 4C and protect it from prolonged exposure to light. DO NOT FREEZE! It is stable for up to 6 months from date of receipt.
Antigen molecule name	CD48
Capture Ab	0.4 mg/mL of mouse anti-CD48 monoclonal antibody. Dilute to a working concentration of 0.5 ug/mL in CBS before coating.
Detection Ab	1.0 mg/mL of rabbit anti-CD48 polyclonal antibody. Dilute to a working concentration of 0.5 ug/mL in detection antibody dilution buffer before use.
Standard	Each vial contains 20 ng of recombinant CD48. Reconstitute standard powder with 1 mL detection antibody dilution buffer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1000 pg/mL is recommended.
Principle of the test	The human CD48 (BCM-1 / SLAMF2) ELISA Pair Set is for the quantitative determination of human CD48. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for CD48 ( BCM-1 / SLAMF2) coated on a 96-well plate. Standards and samples are added tothe wells, and any CD48 (BCM-1 / SLAMF2) present binds to the immobilizedantibody. The wells are washed and a biotinylated rabbit anti-CD48 polyclonalantibody is then added, producing an antibody-antigen-antibody "sandwich". Toproduces color in proportion to the amount of CD48 (BCM-1 / SLAMF2) presentin the sample strepavidin-HRP and TMB substrate solution are loaded. Theabsorbances of the microwell are read at 450 nm.
Streptavidin-HRP	50 uL of streptavidin conjugated to horseradish-peroxidase. 1:2000 Dilution in detection antibody dilution buffer before use.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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