Influenza A H3N2 (A/Brisbane/10/2007) Hemagglutinin / HA ELISA Pair Set

HA

HRP

0.5 mg/mL mouse anti-H3N2 (A/Brisbane/10/2007) HA monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1.0 ug/mL in detection antibody dilution buffer before use

The minimum detectable dose of H3N2 (A/Brisbane/10/2007) HA was determined to be approximately 625 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

The H3N2 (A/Brisbane/10/2007) HA ELISA Pair Set is for the quantitative determination of H3N2 HA .
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for H3N2 ( A/Brisbane/10/2007) HA coated on a 96-well plate. Standards and samples areadded to the wells, and any H3N2 (A/Brisbane/10/2007) HA present binds to theimmobilized antibody. The wells are washed and a horseradish peroxidaseconjugated mouse anti-H3N2 HA monoclonal antibody is then added, producing anantibody-antigen-antibody "sandwich". The wells are again washed and TMBsubstrate solution is loaded, which produces color in proportion to the amountof H3N2 (A/Brisbane/10/2007) HA present in the sample. To end the enzymereaction, the stop solution is added and absorbances of the microwell are readat 450 nm.