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Influenza A H7N7 Hemagglutinin / HA ELISA Pair Set

Influenza A H7N7 Hemagglutinin / HA ELISA Pair Set

ArtNr	SEK11082-5
Hersteller	Sino Biological
Menge	5 Plates
Quantity options	15 Plates 5 Plates
Kategorie	Oncology Antibodies By Cell Type Carcinoma ELISA Immunoassay Immunostaining Antibody Pairs
Typ	ELISA Pair Set
Applikationen	ELISA
Specific against	Flu viruses (Influenza)
Konjugat/Tag	HRP
Sensitivity	The minimum detectable dose of Influenza A H7N7 HA was determined to be approximately 46.8 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	SEK11082
Similar products	HA
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Manufacturer - Type	ELISA
Storage Conditions	Capture Antibody: Aliquot and store at -20C to -80C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20C to -80C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20C to -80C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80C for up to 1 month. Avoid repeated freeze-thaw cycles.
Antigen molecule name	HA
Capture Ab	1.0 mg/mL of rabbit anti-H7N7 HA monoclonal antibody. Dilute to a working concentration of 0.5 ug/mL in CBS before coating.
Detection Ab	0.5 mg/mL rabbit anti-H7N7 HA polyclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 2 ug/mL in detection antibody dilution buffer before use
Standard	Each vial contains 40 ng of recombinant influenza A H7N7 HA. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20C to -80C in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 3 ng/mL is recommended
Principle of the test	The Influenza A H7N7 HA ELISA Pair Set is for the quantitative determination of H7N7 HA. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for InfluenzaA H7N7 HA coated on a 96-well plate. Standards and samples are added to thewells, and any H7N7 HA present binds to the immobilized antibody. The wells arewashed and a horseradish peroxidase conjugated rabbit anti-H7N7 HA polyclonalantibody is then added, producing an antibody-antigen-antibody "sandwich". Thewells are again washed and TMB substrate solution is loaded, which producescolor in proportion to the amount of H7N7 HA present in the sample. To end theenzyme reaction, the stop solution is added and absorbances of the microwellare read at 450 nm.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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