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Influenza A H1N1 (A/Puerto Rico/8/1934) Hemagglutinin / HA ELISA Pair Set

Influenza A H1N1 (A/Puerto Rico/8/1934) Hemagglutinin / HA ELISA Pair Set

ArtNr	SEK11684-5
Hersteller	Sino Biological
Menge	5 Plates
Quantity options	15 Plates 5 Plates
Kategorie	Oncology Antibodies By Cell Type Carcinoma ELISA Immunoassay Immunostaining Antibody Pairs
Typ	ELISA Pair Set
Applikationen	ELISA
Specific against	Flu viruses (Influenza)
Konjugat/Tag	HRP
Sensitivity	The minimum detectable dose of H1N1 (A/Puerto Rico/8/1934) HA was determined to be approximately 46.88 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	SEK11684
Similar products	HA
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Manufacturer - Type	ELISA
Storage Conditions	Capture Antibody: Aliquot and store at -20C to -80C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Store at 4C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE! Standard: Store lyophilized standard at -20C to -80C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80C for up to 1 month. Avoid repeated freeze-thaw cycles.
Antigen molecule name	HA
Capture Ab	0.5 mg/mL of mouse anti-H1N1 (A/Puerto Rico/8/1934) HA monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 ug/mL in PBS before coating.
Detection Ab	0.2 mg/mL mouse anti-H1N1 (A/Puerto Rico/8/1934) HA monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4). Dilute to working concentration of 0.5 ug/mL in detection antibody dilution buffer before use.
Standard	Each vial contains 130 ng of recombinant H1N1 (A/Puerto Rico/8/1934) HA. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20C to -80C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 3 ng/mL is recommended.
Principle of the test	The H1N1 (A/Puerto Rico/8/1934) HA ELISA Pair Set is for the quantitative determination of H1N1 (A/Puerto Rico/8/1934) HA. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solidphase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes amonoclonal antibody specific for H1N1 (A/Puerto Rico/8/1934) HA coated on a96-well plate. Standards and samples are added to the wells, and any H1N1 ( A/Puerto Rico/8/1934) HA present binds to the immobilized antibody. The wellsare washed and a horseradish peroxidase conjugated mouse anti-H1N1(A/Puerto Rico/8/1934) HA monoclonal antibody isthen added, producing an antibody-antigen-antibody "sandwich". The wells areagain washed and TMB substrate solution is loaded, which produces color inproportion to the amount of H1N1 (A/Puerto Rico/8/1934) HA present in thesample. To end the enzyme reaction, the stop solution is added and absorbancesof the microwell are read at 450 nm.

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Menge: 5 Plates

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