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Shiga toxin II subunit B Matched ELISA Antibody Pair Set,E. coli

Shiga toxin II subunit B Matched ELISA Antibody Pair Set,E. coli

ArtNr	SEK40019-15
Hersteller	Sino Biological
Menge	15 Plates
Quantity options	15 Plates 5 Plates
Kategorie	Neuroscience Neural Signaling Antibodies ELISA Immunoassay Immunostaining Antibody Pairs
Typ	ELISA Pair Set
Applikationen	ELISA
Specific against	Escherichia coli (E.coli)
Konjugat/Tag	HRP
Sensitivity	The minimum detectable dose of Enterohemorrhagic E. coli (EHEC) STX2B was determined to be approximately 62.5 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	SEK40019
Similar products	Shiga toxin II subunit B
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Storage Conditions	Capture Antibody: Aliquot and store at -20C to -80C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20C to -80C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20C to -80C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80C for up to 1 month. Avoid repeated freeze-thaw cycles.
Antigen molecule name	Shiga toxin II subunit B
Capture Ab	1.0 mg/mL of mouse anti-E. coli STX2B monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 ug/mL in CBS before coating.
Detection Ab	0.25 mg/mL mouse anti-E. coli STX2B monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % glycerol, pH 7.4). Dilute to working concentration of 0.5 ug/mL in detection antibody dilution buffer before use.
Standard	Each vial contains 19 ng of recombinant E. coli STX2B. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20C to -80C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 4 ng/mL is recommended.
Principle of the test	The Enterohemorrhagic E. coli (EHEC) STX2B ELISA Pair Set is for the quantitative determination of Enterohemorrhagic E. coli (EHEC) STX2B. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for STX2B coated on a 96-well plate. Standards and samples are added to the wells, and any STX2B present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-STX2B monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of STX2B present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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