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Rat E-Selectin/CD62E Matched ELISA Antibody Pair Set

Rat E-Selectin/CD62E Matched ELISA Antibody Pair Set

ArtNr	SEK80033-5
Hersteller	Sino Biological
Menge	5 Plates
Quantity options	15 Plates 5 Plates
Kategorie	Oncology Neuroscience Neural Lineage Markers Molecular Targets for Neuroscience Neural Signaling Neural Adhesion Molecules Antibodies By Cell Type Hematology Leukemia Cell Biology Cell Movement Cell Adhesion ELISA Immunoassay Immunostaining Antibody Pairs
Typ	ELISA Pair Set
Applikationen	ELISA
Specific against	Rat (Rattus norvegicus)
Konjugat/Tag	HRP
Sensitivity	The minimum detectable dose of Rat E-Selectin / CD62e / SELE was determined to be approximately <strong>18.8 pg/ml</strong>. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	sino
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Manufacturer - Type	ELISA
Storage Conditions	Capture Antibody: Aliquot and store at -20C to -80C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20C to -80C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20C to -80C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80C for up to 1 month. Avoid repeated freeze-thaw cycles.
Antigen molecule name	SELE
Capture Ab	0.4 mg/mL of rabbit anti-Rat E-Selectin / CD62e / SELE monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 ug/mL in PBS before coating.
Detection Ab	0.5 mg/mL of rabbit anti-Rat E-Selectin / CD62e / SELE monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % glycerol, pH 7.4). Dilute to working concentration of 0.5 ug/mL in detection antibody dilution buffer before use.
Standard	Each vial contains 33 ng of recombinant Rat E-Selectin / CD62e / SELE. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20C to -80C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1200 pg/mL is recommended.
Principle of the test	The Rat E-Selectin / CD62e / SELE ELISA Pair Set is for the quantitative determination of Rat E-Selectin / CD62e / SELE. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Rat E-Selectin / CD62e / SELE coated on a 96-well plate. Standards and samples are added to the wells, and any Rat E-Selectin / CD62e / SELE present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-Rat E-Selectin / CD62e / SELE monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Rat E-Selectin / CD62e / SELE present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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