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Recombinant Canine c-MET/HGFR Protein (His Tag)

Recombinant Canine c-MET/HGFR Protein (His Tag)

ArtNr	70008-D08H-4
Hersteller	Sino Biological
Menge	4 mg
Quantity options	1 mg 100 ug 2 mg 4 mg 50 ug
Kategorie	Oncology By Cell Type Blastoma Carcinoma Glioma Hematology Lymphoma Myeloma Melanoma Sarcoma Peptides & Proteins Recombinant Proteins Adenocarcinoma Mesothelioma
Typ	Proteins
Specific against	Dog (Canine, Canis lupus familiaris)
Host	Human
Konjugat/Tag	HIS
Purity	> 95 % as determined by SDS-PAGE
Sequence	Met1-Leu935
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	70008-D08H
Similar products	MET
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Manufacturer - Type	Protein
Manufacturer - Conjugate / Tag	C-His
Shipping Temperature	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Storage Conditions	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Antigen molecule name	MET
Protein Construction	A DNA sequence encoding the extracellular domain of canine MET (NP_001002963.1) (Met 1-Leu 935) was expressed, with a polyhistidine tag at the N-terminus.
Expressed Host	Human Cells
Bio-Activity	1. Measured by its binding ability in a functional ELISA. 2. Immobilized human HGF (Cat:10463-HNAS) at 10 ug/mL (100 ul/well) can bind canine c-MET (Cat:70008-D08H), The EC50 of canine c-MET (Cat:70008-D08H) is 7 ng/mL. 3. Immobilized canine MET-His at 10 ug/ml (100 ul/well) can bind biotinylated human HGF-His (Cat:10463-H08H), The EC50 of biotinylated human HGF-His (Cat:10463-H08H) is 0.11-0.25 ug/ml.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Glu 25
Molecule Mass	The recombinant canine c-Met is a disulfide-linked heterodimer composed of proteolytically cleaved alpha and beta subunits. Each alpha and beta subunit together consists of 922 amino acids and has a predicted molecular mass of 103 (alpha =33 + beta =70) kDa. As a result of glycosylation, the apparent molecular mass of the canine c-Met is approximately 42-47 kDa and 85-95 kDa respectively in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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70008-D08H-4-datasheet.pdf

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Menge: 4 mg

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