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Recombinant Human CD146/MCAM Protein (His Tag) Europäischer Partner

ArtNr 10115-H08H-4
Hersteller Sino Biological
Menge 4 mg
Quantity options 1 mg 100 ug 2 mg 4 mg
Kategorie
Typ Proteins
Specific against Human (Homo sapiens)
Host Human
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias CD146, MUC18
Similar products MCAM
Lieferbar
Manufacturer - Category
Protein
Molecule
MCAM
Synonym
CD146, MUC18
Storage
Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Protein Construction
A DNA sequence encoding the extracellular domain of human CD146 precursor (NP_006491.2) (Met 1-Gly 559) was expressed with a C-terminal polyhistidine tag.
Expressed Host
Human Cells
Purity
> 98 % as determined by SDS-PAGE
Bio-Activity
Measured by the ability of the immobilized protein to support the adhesion of the HUVEC human umbilical vein endothelial cell line . When 3 x 10E4 cells/well are added to human CD146 coated plates (0.8 ug/ml, 100 ul/well), approximately >50 % will adhere after one hour at 37 C.
Endotoxin
< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Val 24
Molecule Mass
The recombinant CD146 comprises 547 amino acids and predicts a molecular mass of 61.3 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 80-90 kDa band in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4
Accession
NP_006491.2
Amino acid sequence
Met1-Gly559
TAG
C-His

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 4 mg
Lieferbar: In stock
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