Recombinant Human ACVR2B Protein (ECD, hFc Tag), HPLC-verified

C-human IgG1-Fc

Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

A DNA sequence encoding the N-terminal segment (Met 1-Thr 134) from the extracellular domain of human ACVR2B (NP_001097.2) was expressed with the fused human IgG1 Fc region at the C-terminus.

1. Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 0.02-0.1 ug/mL in the presence of 10 ng/mL recombinant Activin A.
2. Measured by its binding ability in a functional ELISA.
3. Immobilized human ACVR2B (Cat:10229-H02H) at 10 ug/mL (100 ul/well) can bind biotinylated human INHBA-His (Cat:10429-H08H), The EC50 of biotinylated human INHBA-His (Cat:10429-H08H) is 0.112 ug/mL.
4. Immobilized human ACVR2B (Cat:10229-H02H) at 10 ug/mL (100 ul/well) can bind biotinylated mouse INHBA-His (Cat:Cat:50659-M08H), The EC50 of biotinylated mouse INHBA-His (Cat:Cat:50659-M08H) is 0.161 ug/mL.

Ser 19

Lyophilized from sterile PBS, pH 7.4