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Recombinant Human Osteoprotegerin/TNFRSF11B Protein (His Tag) Europäischer Partner

ArtNr 10271-H08H-1
Hersteller Sino Biological
Menge 1 mg
Quantity options 1 mg 100 ug 2 mg 4 mg
Kategorie
Typ Proteins
Specific against Human (Homo sapiens)
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias MGC29565, OCIF, OPG, PDB5, TR1
Similar products TNFRSF11B
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Manufacturer - Category
Protein
Molecule
TNFRSF11B
Synonym
MGC29565, OCIF, OPG, PDB5, TR1
Storage
Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Protein Construction
A DNA sequence encoding the human TNFRSF11B (NP_002537.3) (Met 1-Leu 401) was fused with a polyhistidine tag at the C-terminus.
Expressed Host
Human Cells
Purity
> 97 % as determined by SDS-PAGE
Bio-Activity
1. Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L-929 mouse fibroblast cells treated with TRAIL. The ED50 for this effect is typically 5-20 ng/mL in the presence of 20 ng/mL Recombinant Human TRAIL/TNFSF10 (Catalog # 10409-HNAE).
2. Measured by its binding ability in a functional ELISA. Immobilized human TNFRSF11B-His at 10 ug/ml (100 ul/well) can bind human Fc-TNFSF11 (Cat?11682-H01H) with a linear ranger of 3.125-200 ng/mL.
Endotoxin
< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Glu 22
Molecule Mass
The secreted recombinant human TNFRSF11B comprises 391 amino acids and has a predicted molecular mass of 45.3 kDa. As a result of glycosylation, rhTNFRSF11B migrates as an approximately 55 kDa bnd in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4
Accession
NP_002537.3
Amino acid sequence
Met1-Leu401
TAG
C-His

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 1 mg
Lieferbar: In stock
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