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Recombinant Human Serpin A1/Alpha-1-antitrypsin Protein (His Tag), HPLC-verified Europäischer Partner

ArtNr 10306-H08H-1
Hersteller Sino Biological
Menge 1 mg
Quantity options 1 mg 100 ug 2 mg 4 mg
Kategorie
Typ Proteins
Specific against Human (Homo sapiens)
Host Human
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias A1A, A1AT, AAT, alpha1AT, MGC23330, MGC9222, PI, PI1, PRO2275, SerpinA1
Similar products SERPINA1
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Manufacturer - Category
Protein
Molecule
SerpinA1
Synonym
A1A, A1AT, AAT, alpha1AT, MGC23330, MGC9222, PI, PI1, PRO2275, SerpinA1
Storage
Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Protein Construction
A DNA sequence encoding the human SerpinA1 (NP_000286.3) pre-protein (Met 1-Lys 418) was expressed with a C-terminal polyhistidine tag.
Expressed Host
Human Cells
Purity
> 97 % as determined by SDS-PAGE
Bio-Activity
Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Anaspec, Catalog#27114) . The IC50 value is < 3.0 nM, as measured in 100uL reaction mixture containing 1.25 ng trypsin (Sigma, Catalog#T1426), 10 uM substrate, 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, pH 7.5.
Endotoxin
< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Glu 25
Molecule Mass
The secreted mature form of recombinant human SerpinA1 consists of 405 amino acids and has a calculated molecular mass of 45.7 kDa. Due to glycosylation, the rhSerpinA1 migrates as an approximately 55-60 kDa protein in SDS-PAGE analysis under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4
Accession
NP_000286.3
Amino acid sequence
Met1-Lys418
TAG
C-His

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 1 mg
Lieferbar: In stock
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