Human HMGB1 / HMG1 Protein (Fc Tag)

Hersteller Sino Biological
Kategorie
Typ Proteins
Specific against Human
Format Human Cell
Menge 20 ug
Host Human
ArtNr 10326-H01H-20
Eclass 6.1 34160400
Eclass 9.0 42020190
Lieferbar
Molecule
HMGB1
Synonym
HMG-1, HMG1, HMG3, SBP-1
Storage
Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Protein Construction
A DNA sequence encoding the human HMGB1 protein (NP_002119.1) (Met 1-Glu 215) was expressed with the fused Fc region of human IgG1 at the N-terminus.
Expressed Host
Human Cells
Purity
> 97 % as determined by SDS-PAGE
Bio-Activity
Measured by its binding ability in a functional ELISA . Immobilized recombinant mouse AGER at 2 ug/ml (100 ul/well) can bind human HMGB1. The EC50 of human HMGB1 is 0.23 ug/ml .
Endotoxin
< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Glu 20
Molecule Mass
The recombinant human HMGB1/Fc is a disulfide-linked homodimeric protein.The reduced monomer consists of 452 amino acids and has a predictedmolecular mass of 51.5 kDa. As a result of glycosylation, the apparent molecular mass of rhHMGB1/Fc monomer is approximately 55-60 kDa inSDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5
Accession
NP_002119.1
Amino acid sequence
Met1-Glu215
TAG
N-human IgG1-Fc
Menge: 20 ug
Lieferbar: In stock
Listenpreis: 192,96 €
Preis: 192,96 €
lieferbar

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