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Recombinant Human ICAM-1/CD54 Protein (ECD, His & hFc Tag), HPLC-verified Europäischer Partner

ArtNr 10346-H03H-4
Hersteller Sino Biological
Menge 4 mg
Quantity options 1 mg 100 ug 2 mg 4 mg 50 ug
Kategorie
Typ Proteins
Specific against Human (Homo sapiens)
Host Human
Konjugat/Tag HIS, Fc
Purity > 95 % as determined by SDS-PAGE
Sequence Met1-Glu480
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias BB2,CD54,ICAM-1,P3.58
Similar products ICAM1
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Manufacturer - Type
Protein
Manufacturer - Conjugate / Tag
C-human IgG1-Fc & His
Shipping Temperature
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Storage Conditions
Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Antigen molecule name
ICAM1
Protein Construction
A DNA sequence encoding the human ICAM1 (NP_000192.2) extracellular domain (Met 1-Glu 480) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Expressed Host
Human Cells
Bio-Activity
Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells.
When 5 x 10E4 cells/well are added to Recombinant Human ICAM-1/CD54 coated plates (12.5 ug/mL with 100 uL/well), >40% cells will adhere after 1 hour of incubation at 37C.
Endotoxin
< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Gln 28
Molecule Mass
The recombinant human ICAM1/Fc is a disulfide-linked homodimer. The reduced monomer consists of 700 amino acids and has a predicted molecular mass of 77.4 kDa. As a result of glycosylation, the apparent molecular mass of rh ICAM1/Fc monomer migrates with an apparent molecular mass of 100-110 kDa in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 4 mg
Lieferbar: In stock
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