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Recombinant Human Sonic Hedgehog/SHH Protein (aa 1-197, His Tag)

Recombinant Human Sonic Hedgehog/SHH Protein (aa 1-197, His Tag)

ArtNr	10372-H08H1-20
Hersteller	Sino Biological
Menge	20 ug
Quantity options	1 mg 100 ug 2 mg 20 ug 4 mg
Kategorie	Oncology Neuroscience Neural Signaling By Cell Type Blastoma Sarcoma Peptides & Proteins Recombinant Proteins
Typ	Proteins
Specific against	Human (Homo sapiens)
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	HHG1, HLP3, HPE3, MCOPCB5, SMMCI, Sonic hedgehog, TPT, TPTPS
Similar products	SHH
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Manufacturer - Category	Protein
Molecule	SHH
Synonym	HHG1, HLP3, HPE3, MCOPCB5, SMMCI, Sonic hedgehog, TPT, TPTPS
Storage	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Protein Construction	A DNA sequence encoding the amino acid sequence (Met 1-Gly 197) of human SHH (Q15465), that is Sonic hedgehog protein N-product, was fused with a polyhistidine tag at the C-terminus.
Expressed Host	Human Cells
Purity	> 95 % as determined by SDS-PAGE
Bio-Activity	Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun.237:465. The ED50 for this effect is typically 2-10 ug/mL.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Cys 24
Molecule Mass	The recombinant human SHH (aa 1-197) consists of 185 amino acids and has a predicted molecular mass of 21 kDa. The apparent molecular mass of the protein is approximately 24 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from sterile PBS, pH 7.4
Accession	Q15465
Amino acid sequence	Met1-Gly197
TAG	C-His

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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10372-H08H1-20-datasheet.pdf

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Menge: 20 ug

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