Recombinant Human IGF1 Protein, HPLC-verified

Native

Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

A DNA sequence encoding the mature form of human IGF1 isoform 1 (P05019-1) (Gly 49-Ala 118) was expressed and purified.

1. Measured by its ability to bind human IGFBP4-His (Cat:10967-H08H) in a functional ELISA.
2. Measured by its ability to bind mouse IGFBP4-His (Cat:50250-M08H) in a functional ELISA.
3. Measured by its ability to bind Cynomolgus IGFBP1-His (Cat:90085-C08H) in a functional ELISA.
4. Measured by its ability to bind human IGF1R (aa 954-1367)-GST (Cat:10164-H20B1) in a functional ELISA.
5. Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is typically 0.5-2 ng/mL.
6. Measured in a cell proliferation assay using MCF-7 cells. The ED50 for this effect is typically 1-4 ng/mL.

Met

Lyophilized from sterile 30% Acetonitrile, 0.1% TFA