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Recombinant Human TIE-2 Protein (ECD, His & hFc Tag), HPLC-verified Europäischer Partner

ArtNr 10700-H03H-1
Hersteller Sino Biological
Menge 1 mg
Quantity options 1 mg 2 mg 200 ug 4 mg
Kategorie
Typ Proteins
Specific against Human (Homo sapiens)
Host Human
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias CD202B, TIE-2, TIE2, VMCM, VMCM1
Similar products TEK
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Manufacturer - Category
Protein
Molecule
TEK
Synonym
CD202B, TIE-2, TIE2, VMCM, VMCM1
Storage
Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Protein Construction
A DNA sequence encoding the extracellular domain (Met 1-Lys 745) of human Tie2 (NP_000450.2) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Expressed Host
Human Cells
Purity
> 90 % as determined by SDS-PAGE
Bio-Activity
Measured by its binding ability in a functional ELISA . Immobilized recombinant human Angiopoietin-2 at 10 ug/ml (100 ul/well) can bind Human Tie2 / Fc chimera with a range of 0.2-20 ug/ml.
Endotoxin
< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Ala 23
Molecule Mass
The recombinant human Tie2/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 970 amino acids and predicts a molecular mass of 108.5 kDa. As a result of glycosylation, the apparent molecular mass of rhTie2/Fc monomer is approximately 125-135 kDa in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5
Accession
NP_000450.2
Amino acid sequence
Met1-Lys745
TAG
C-human IgG1-Fc & His

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 1 mg
Lieferbar: In stock
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