Human ENTPD3 / NTPDase3 / CD39L3 Protein (His Tag)

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Hersteller Sino Biological
Kategorie
Typ Proteins
Specific against Human
Menge 2 mg
Host Baculovirus
ArtNr 10909-H08B-2
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Molecule
ENTPD3
Synonym
CD39L3, HB6, NTPDase-3
Storage
Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Protein Construction
A DNA sequence encoding the human ENTPD3 (O75355) extracellular domain (Gln 44-Pro 485) was fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.
Expressed Host
Baculovirus-Insect Cells
Purity
> 97 % as determined by SDS-PAGE
Bio-Activity
Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-triphosphate (ATP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit (Catalog # DY996).
The specific activity is >70, 000 pmol/min/ug.
Endotoxin
< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Gln 44
Molecule Mass
The recombinant human ENTPD3 (aa44-485) consists of 452 amino acids and predicts a molecular mass of 51 kDa. It migrates as an approximately 50 KDa band in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly
Accession
O75355
Amino acid sequence
Gln44-Pro485
TAG
C-His

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Menge: 2 mg
Lieferbar: In stock
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