Human R-Spondin 1 / RSPO1 Protein (His Tag)

CRISTIN3, R-Spondin 1, RSPO

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Human Cells

1. Measured by its binding ability in a functional ELISA. Immobilized human RSPO1 at 20 ug/ml (100 ul/well) can bind human LIMPII with a linear ranger of 32-800 ng/ml.
2. Measured by its binding ability in a functional ELISA. Immobilized human RSPO1 at 20 ug/ml (100 ul/well) can bind mouse CD36 with a linear ranger of 6.4-800 ng/ml.
3. Measured by its ability to induce activation of ßcatenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 0.05-0.5 ug/mL in the presence of 5 ng/mL recombinant mouse Wnt3a.

Ser 21

Lyophilized from sterile PBS, pH 7.4

Met1-Ala263