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Recombinant Human IL-23 Protein (His Tag)

Recombinant Human IL-23 Protein (His Tag)

ArtNr	CT012-H08H-2
Hersteller	Sino Biological
Menge	2 mg
Quantity options	1 mg 100 ug 2 mg 4 mg 5 ug
Kategorie	Peptides & Proteins Recombinant Proteins Cell Biology Cell Culture Cytokines
Typ	Proteins
Specific against	Human (Homo sapiens)
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	IL-23, IL-23A, IL23P19, P19, SGRF
Similar products	IL23
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Manufacturer - Category	Protein
Molecule	IL23
Synonym	IL-23, IL-23A, IL23P19, P19, SGRF
Storage	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Protein Construction	A DNA sequence encoding the IL23A (Q9NPF7) (Met 1-Pro 189) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the p40 subunit of human IL12, termed as IL12B (NP_002178.2) (Met 1-Ser 328) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the IL23 heterodimer was purified.
Expressed Host	Human Cells
Purity	> 90 % as determined by SDS-PAGE
Bio-Activity	1. Measured by its ability to bind biotinylated recombinant human IL12RB1 in a functional ELISA. 2. Measured by its binding ability in a functional ELISA. Immobilized human IL23A-His+IL12B-His at 10 ug/ml (100 ul/well) can bind human IL23R-Fc (Cat:13840-H02H). The EC50 of human IL23R-Fc (Cat:13840-H02H) is 0.28-0.66 ug/ml. 3.Measured by its binding ability in a functional ELISA. Immobilized human IL23A-His+IL12B-His at 10 ug/ml (100 ul/well) can bind Cynomolgus IL23R-Fc (Cat:90123-C02H). The EC50 of Cynomolgus IL23R-Fc (Cat:90123-C02H) is 0.14-0.35 ug/ml. 4. Measured by its ability to induce IL17 secretion by mouse splenocytes. The ED50 for this effect is 0.5-2.5ng/mL.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Arg 20 & Ile 23
Molecule Mass	The recombinant IL23 heterodimer of human IL23A/IL12B comprises 498 (181 + 317) amino acids and has a calculated molecular mass of 56.3 (20.1 + 36.2) kDa. The apparent molecular mass of rhIL23 heterodimer is approximately 22 & 45 kDa respectively in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4
Accession	Q9NPF7 & NP_002178.2
Amino acid sequence	Met1-Pro189 & Met1-Ser328
TAG	C-His

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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CT012-H08H-2-datasheet.pdf

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Menge: 2 mg

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