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Recombinant Human CD3D & CD3E Heterodimer Protein (ECD), HPLC-verified

Recombinant Human CD3D & CD3E Heterodimer Protein (ECD), HPLC-verified

ArtNr	CT026-H0323H-50
Hersteller	Sino Biological
Menge	50 ug
Quantity options	1 mg 2 mg 4 mg 50 ug 500 ug
Kategorie	Oncology By Cell Type Hematology Leukemia Lymphoma Peptides & Proteins Recombinant Proteins
Typ	Proteins
Format	Human Cell
Specific against	Human (Homo sapiens)
Host	Human
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	CD3-DELTA, IMD19, T3D
Similar products	CD3D & CD3E
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Manufacturer - Category	Protein
Molecule	CD3D & CD3E
Synonym	CD3-DELTA, IMD19, T3D
Storage	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Protein Construction	A DNA sequence encoding the extracellular domain (Met 1-Ala 105) of human CD3D (P04234) was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met 1-Asp 126) of human CD3E (P07766) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD3D/CD3E heterodimer was purified.
Expressed Host	Human Cells
Purity	> 90 % as determined by SDS-PAGE
Bio-Activity	Measured by its binding ability in a functional ELISA. Immobilized human CD3D & CD3E Heterodimer Protein (Cat:CT026-H0323H) at 10 ug/ml (100 ul/well) can bind OKT3-mIgG2a. The EC50 of OKT3-mIgG2a is 0.07-0.17 ug/ml.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Phe 22 & Asp 23
Molecule Mass	The recombinant heterodimer of human CD3D/CD3E comprises 670 (333+354) amino acids and has a calculated molecular mass of 77.5 (37.5+40? kDa. As a result of glycosylation, the apparent molecular mass of human CD3D/CD3E heterodimer is approximately 45-50 kDa in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4
Accession	P04234 & P07766
Amino acid sequence	Met1-Ala105 & Met1-Asp126
TAG	C-human IgG1-Fc & His & C-human IgG1-Fc & Flag

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 50 ug

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