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Recombinant Mouse GFR Alpha-1/GFRA1 Protein (His Tag) Europäischer Partner

ArtNr 50171-M08H-50
Hersteller Sino Biological
Menge 50 ug
Quantity options 1 mg 100 ug 2 mg 4 mg 50 ug
Kategorie
Typ Proteins
Specific against Mouse (Murine, Mus musculus)
Konjugat/Tag HIS
Purity > 98 % as determined by SDS-PAGE
Sequence Met1-Ser425
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias AU042498
Similar products GFRA1
Lieferbar
Manufacturer - Conjugate / Tag
C-His
Shipping Temperature
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Storage Conditions
Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Antigen molecule name
GFRA1
Protein Construction
A DNA sequence encoding the extracellular domain (Met 1-Ser 425) of mouse GFRalpha1 isoform beta (AAB86600.1) was expressed with a C-terminal polyhistidine tag.
Expressed Host
Human Cells
Bio-Activity
1. Measured in a cell proliferation assay using SH?SY5Y human neuroblastoma cells.
The ED50 for this effect is typically 0.3-1.5 ug/mL in the presence of 40 ng/mL Recombinant Human GDNF.
2. Measured by its ability to bind with human GDNF (Cat:10561-HNCH) in a functional ELISA.
Endotoxin
< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Asp 25
Molecule Mass
The secreted recombinant mouse GFRalpha1consists of 412 amino acids and has a calculated molecular mass of 46.3 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 55-60 kDa protein in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 50 ug
Lieferbar: In stock
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