Mouse CD73 / NT5E Protein (His Tag)

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Hersteller Sino Biological
Kategorie
Typ Proteins
Specific against Mouse
Menge 4 mg
Host Human
ArtNr 50231-M08H-4
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Molecule
NT5E
Synonym
2210401F01Rik, AI447961, CD73, eNT, NT, Nt5
Storage
Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Protein Construction
A DNA sequence encoding the mouse NT5E (NP_035981.1) without the propeptide, corresponding to the amino acid (Met 1-Lys 549) was expressed, with a C-terminal polyhistidine tag.
Expressed Host
Human Cells
Purity
> 95 % as determined by SDS-PAGE
Bio-Activity
Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit (Catalog # DY996).
The specific activity is >10, 000 pmol/min/ug.
Endotoxin
< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Trp 29
Molecule Mass
The secreted recombinant mouse NT5E consists of 532 amino acids and has a calculated molecular mass of 59.4 kDa which is also detected in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4
Accession
NP_035981.1
Amino acid sequence
Met1-Lys549
TAG
C-His

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Menge: 4 mg
Lieferbar: In stock
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