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Recombinant Mouse Src Protein (His & GST Tag)

Recombinant Mouse Src Protein (His & GST Tag)

ArtNr	50311-M20B-2
Hersteller	Sino Biological
Menge	2 mg
Quantity options	1 mg 2 mg 20 ug 4 mg 50 ug
Kategorie	Oncology By Cell Type Blastoma Carcinoma Glioma Hematology Lymphoma Myeloma Melanoma Sarcoma Peptides & Proteins Recombinant Proteins Adenocarcinoma
Typ	Proteins
Specific against	Mouse (Murine, Mus musculus)
Host	Baculovirus-Infected Insect Cells
Konjugat/Tag	GST, HIS
Purity	> 95 % as determined by SDS-PAGE
Sequence	Met1-Leu535
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	AW259666,pp60c-src
Similar products	SRC
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	Protein
Manufacturer - Conjugate / Tag	N-GST & His
Shipping Temperature	Blue ice shipping
Storage Conditions	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Antigen molecule name	SRC
Protein Construction	A DNA sequence encoding the mouse SRC (NP_001020566.111) (Met 1-Leu 535) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Expressed Host	Baculovirus-Insect Cells
Bio-Activity	1. The specific activity was determined to be 166 nmol/min/mg using poly [Glu, Tyr] 4:1 as substrate. 2. Measured by its binding ability in a functional ELISA. Immobilized recombinant Mouse SRC at 2 ug/ml (100 ul/well) can bind biotinylated human PTPRA (aa 174-793) with a linear range of 0.032-0.8 ug/ml.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Met
Molecule Mass	The recombinant mouse SRC/GST chimera consists of 773 amino acids and has a calculated molecular mass of 87.7 kDa. It migrates as an approximately 80 kDa band in SDS-PAGE under reducing conditions.
Formulation	Supplied as sterile 20mM Tris, 500mM NaCl, 10% gly, pH 8.0

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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50311-M20B-2-datasheet.pdf

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Menge: 2 mg

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