Recombinant Mouse TNFR1/CD120a Protein (His Tag), HPLC-verified

Protein

CD120a, FPF, p55, p55-R, TNF-alphaR1, TNF-R, TNF-R-I, TNF-R1, TNF-R55, TNFalpha-R1, TNFAR, Tnfr-2, Tnfr1, TNFR60, TNFRI, TNFRp55

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Human Cells

1. Measured by its binding ability in a functional ELISA.
2. Immobilized human TNFa at 10 ug/mL (100 ul/well) can bind biotinylated mouse TNFRSF1A-his. The EC50 of biotinylated mouse TNFRSF1A-his is 0.28 ug/mL.
3. Immobilized mouse TNFa at 10 ug/mL (100 ul/well) can bind biotinylated mouse TNFRSF1A-his. The EC50 of biotinylated mouse TNFRSF1A-his is 0.13 ug/mL.
4. Measured by its ability to inhibit the TNFalpha-mediated cytotoxicity in the L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 0.5-1.5 ug/mL in the presence of 0.1 ng/mL recombinant mouse TNFalpha.

Leu 30

Lyophilized from sterile PBS, pH 7.4

Met1-Ala212