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Recombinant Mouse DLL4 Protein (His Tag), HPLC-verified

Recombinant Mouse DLL4 Protein (His Tag), HPLC-verified

ArtNr	50640-M08H-1
Hersteller	Sino Biological
Menge	1 mg
Quantity options	1 mg 100 ug 2 mg 4 mg 50 ug
Kategorie	Oncology Neuroscience Molecular Targets for Neuroscience By Cell Type Carcinoma Peptides & Proteins Recombinant Proteins
Typ	Proteins
Specific against	Mouse (Murine, Mus musculus)
Host	Human
Konjugat/Tag	HIS
Purity	> 96 % as determined by SDS-PAGE
Sequence	Met1-Pro525
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	Delta4
Similar products	DLL4
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Manufacturer - Type	Protein
Manufacturer - Conjugate / Tag	C-His
Shipping Temperature	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Storage Conditions	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Antigen molecule name	DLL4
Protein Construction	A DNA sequence encoding the extracellular domain of mouse DLL4 (NP_062327.2) (Met 1-Pro 525) was expressed, with a C-terminal polyhistidine tag.
Expressed Host	Human Cells
Bio-Activity	1. Measured by its binding ability in a functional ELISA. Immobilized mouse DLL4-his (Cat:50640-M08H) at 10 ug/mL (100 uL/well) can bind biotinylated mouse NOTCH1-his. The EC50 of biotinylated mouse NOTCH1-his is 30 ng/mL. 2. Measured by the ability of the immobilized protein to enhance BMP2-induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 0.2-3 ug/mL.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Ser 28
Molecule Mass	The secreted recombinant mouse DLL4 comprises 509 amino acids and has a calculated molecular mass of 55.7 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 68 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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50640-M08H-1-datasheet.pdf

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Menge: 1 mg

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