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Recombinant Mouse IL-12 Protein (hFc Tag)

Recombinant Mouse IL-12 Protein (hFc Tag)

ArtNr	CT021-M02H-1
Hersteller	Sino Biological
Menge	1 mg
Quantity options	1 mg 2 mg 20 ug 4 mg 50 ug
Kategorie	Peptides & Proteins Recombinant Proteins Cell Biology Cell Culture Cytokines
Typ	Proteins
Specific against	Mouse (Murine, Mus musculus)
Host	Human
Konjugat/Tag	Fc
Purity	> 85 % as determined by SDS-PAGE
Sequence	Met1-Ala215 & Met1-Ser335
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	Il-12a,Il-12b,IL-12p35,Il-12p40,Il12p40,Ll12a,p35,p40
Similar products	IL12A & IL12B
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Manufacturer - Type	Protein
Manufacturer - Conjugate / Tag	C-human IgG1-Fc
Shipping Temperature	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Storage Conditions	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Antigen molecule name	IL12A & IL12B
Protein Construction	A DNA sequence encoding the p35 subunit of mouse IL12, termed as IL12A (P43431) (Met 1-Ala 215) was fused with the Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the p40 subunit of mouse IL12, termed as IL12B (P43432) (Met 1-Ser 335) was fused with the Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.
Expressed Host	Human Cells
Bio-Activity	1. Immobilized mouse mIL12A+mIL12B at 10 ug/ml (100 ul/well) can bind biotinylated human IL12RB1-His (Cat:11674-H08H), The EC50 of biotinylated human IL12RB1-His (Cat:11674-H08H) is 38.7-90.3 ng/ml. 2. Measured in a cell proliferation assay using Anti-CD3-stimulated PBMC. The ED50 for this effect is typically 0.2-0.8 ng/mL.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Arg 23 & Met 23
Molecule Mass	The recombinant mouse IL12 heterodimer of IL12A/IL12B, both was Fc chimera, comprises 988 (434 + 554) amino acids and has a calculated molecular mass of 111.5 (48.7 + 62.8) kDa. The apparent molecular mass of rh IL12 heterodimer is approximately 55 & 75 kDa respectively in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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CT021-M02H-1-datasheet.pdf

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Menge: 1 mg

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