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MERS-CoV Spike Protein (S1+S2 ECD, aa 1-1297, His Tag)

MERS-CoV Spike Protein (S1+S2 ECD, aa 1-1297, His Tag)

ArtNr	40069-V08B-100
Hersteller	Sino Biological
Menge	100 ug
Quantity options	1 mg 100 ug 2 mg 4 mg
Kategorie	Neuroscience Aging & Neurological Disorders Infectious Diseases Viral Infections Coronavirus (COVID-19) Peptides & Proteins Recombinant Proteins Flow Cytometry ELISA
Typ	Proteins
Format	Baculovirus-Insect cells
Applikationen	FC, ELISA
Specific against	MERS-CoV
Host	Baculovirus-Infected Insect Cells
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	S
Similar products	Spike
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Manufacturer - Category	Protein
Molecule	Spike
Synonym	S
Storage	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Protein Construction	A DNA sequence encoding the extracellular domain of spike protein (Human betacoronavirus 2c EMC/2012)( AFS88936.1) (Met1-Trp1297) was fused with a polyhistidine tag at the C-terminus.
Expressed Host	Baculovirus-Insect Cells
Purity	> 85 % as determined by SDS-PAGE
Bio-Activity	Measured by its binding ability in a functional ELISA. 1. Immobilized Spike (HCoV-EMC/2012) (ECD, aa 1-1297) (Cat: 40069-V08B) at 10 ug/ml (100 ul/well) can bind biotinylated human ACE2-Fc (Cat: 10108-H02H). The EC50 of biotinylated human ACE2-Fc (Cat: 10108-H02H) is 0.9-1.4 ug/ml. 2. Immobilized Spike (HCoV-EMC/2012) (ECD, aa 1-1297) (Cat: 40069-V08B) at 10 ug/ml (100 ul/well) can bind biotinylated DPP4 (Cat: 10688-HNCH). The EC50 of of biotinylated DPP4 (Cat: 10688-HNCH) is 0.02-0.04 ug/ml. 3. Immobilized Spike (HCoV-EMC/2012) (ECD, aa 1-1297) (Cat: 40069-V08B) at 10 ug/ml (100 ul/well) can bind biotinylated Fc-DPP4 (Cat: 10688-H01H). The EC50 of biotinylated Fc-DPP4 (Cat: 10688-H01H) is 0.01-0.02 ug/ml.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Tyr 18
Molecule Mass	The recombinant extracellular domain of spike protein (Human betacoronavirus 2c EMC/2012) comprises 1289 amino acids and has a predicted molecular mass of 142.2 kDa. It migrates as an approximately 157 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris, 500mM Nacl, pH 7.4, 10% glycerol.
Accession	AFS88936.1
Amino acid sequence	Met1-Trp1297
TAG	C-His

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 100 ug

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