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Recombinant Rat GDNF Protein (His Tag)

Recombinant Rat GDNF Protein (His Tag)

ArtNr	80050-R07B-10
Hersteller	Sino Biological
Menge	10 ug
Quantity options	1 mg 10 ug 2 mg 20 ug 4 mg 50 ug
Kategorie	Neuroscience Molecular Targets for Neuroscience Peptides & Proteins Recombinant Proteins
Typ	Proteins
Specific against	Rat (Rattus norvegicus)
Host	Baculovirus-Infected Insect Cells
Konjugat/Tag	HIS
Purity	> 95 % as determined by SDS-PAGE
Sequence	Ser78-Ile211
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	80050-R07B
Similar products	GDNF
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Manufacturer - Conjugate / Tag	N-His
Shipping Temperature	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Storage Conditions	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Antigen molecule name	GDNF
Protein Construction	A DNA sequence encoding the mature form of rat GDNF (Q07731-1) (Ser78-Ile211) was expressed, with a polyhistide tag at the N-terminus.
Expressed Host	Baculovirus-Insect Cells
Bio-Activity	1. Measured in a cell proliferation assay using SH?SY5Y human neuroblastoma cells. The ED50 for this effect is typically 10-50 ng/mL in the presence of Recombinant Rat GFR alpha?1/GDNF R alpha?1 His Chimera. 2. Immobilized rat His-GDNF (78-211) at 10 ug/ml (100 ul/well) can bind rat GFRA1-Fc (cat:80021-R02H), The EC50 of rat GFRA1-Fc (cat:80021-R02H) is 20-46.6 ng/ml.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	His
Molecule Mass	The recombinant rat GDNF consists of 150 amino acids and predicts a molecular mass of 17.1 KDa. It migrates as an approximately 20 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 10 ug

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