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Recombinant Rat TNFR1/CD120a Protein (hFc Tag)

Recombinant Rat TNFR1/CD120a Protein (hFc Tag)

ArtNr	80181-R02H-100
Hersteller	Sino Biological
Menge	100 ug
Quantity options	1 mg 100 ug 2 mg 4 mg 50 ug
Kategorie	Metabolism Diabetes Glucose Homeostasis Peptides & Proteins Recombinant Proteins Cell Biology Obesity Cell Status Necrosis Apoptosis
Typ	Proteins
Specific against	Rat (Rattus norvegicus)
Host	Human
Konjugat/Tag	Fc
Purity	> 90 % as determined by SDS-PAGE
Sequence	Met1-Ala211
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	80181-R02H
Similar products	TNFRSF1A
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Manufacturer - Type	Protein
Manufacturer - Conjugate / Tag	C-human IgG1-Fc
Shipping Temperature	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Storage Conditions	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Antigen molecule name	TNFRSF1A
Protein Construction	A DNA sequence encoding the rat TNFRSF1A (P22934) (Met1-Ala211) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
Expressed Host	Human Cells
Bio-Activity	1. Measured by its ability to inhibit TNFalpha-mediated cytotoxicity in L-929 mouse fibroblast cells in the presence of metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30 ng/mL in the presence of 0.05 ng/mL of ratTNFalpha. 2. Immobilized mouse TNFa (80-235) (Cat:50349-MNAE ) at 10 ug/ml (100 ul/well) can bind rat TNFRSF1A-Fc, The EC50 of rat TNFRSF1A-Fc is 0.08-0.20 ug/ml.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Ile 22
Molecule Mass	The recombinant rat TNFRSF1A/Fc is a disulfide-linked homodimer. The reduced monomer comprises 431 amino acids and has a predicted molecular mass of 47.9 kDa. The apparent molecular mass of the protein is approximately 59 kDa in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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80181-R02H-100-datasheet.pdf

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Menge: 100 ug

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