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Recombinant Rat IFN gamma Protein (hFc Tag) Europäischer Partner

ArtNr 80234-R02H-100
Hersteller Sino Biological
Menge 100 ug
Quantity options 1 mg 100 ug 2 mg 4 mg
Kategorie
Typ Proteins
Specific against Rat (Rattus norvegicus)
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias 80234-R02H
Similar products IFNG
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Manufacturer - Category
Protein
Molecule
IFNG
Storage
Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Protein Construction
A DNA sequence encoding the rat IFNG (P01581) (Met 1-Cys 156) was fused with the Fc region of human IgG1 at the C-terminus.
Expressed Host
Human Cells
Purity
> 92 % as determined by SDS-PAGE
Bio-Activity
1. Measured in antiviral assay using L929 cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is typically 1-5 ng/mL.
2. Measured by its binding ability in a functional ELISA. Immobilized rat IFNG-Fc (cat:80234-R02H) at 10 ug/ml (100 ul/well) can bind biotinylated rat IFNGR-Fc (Cat:80182-R02H). The EC50 of biotinylated rat IFNGR-Fc (Cat:80182-R02H) is 20.1-46.9 ng/ml.
Endotoxin
< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Gln 23
Molecule Mass
The secreted recombinant rat IFNG/Fc is a disulfide-linked homodimer. The reduced monomer comprises 375 amino acids and predicts a molecular mass of 42.5 kDa. The apparent molecular mass of the rat IFNG/Fc monomer is approximately 52 kDa in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4
Accession
P01581
Amino acid sequence
Met1-Cys156
TAG
C-human IgG1-Fc

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 ug
Lieferbar: In stock
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