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Human coronavirus HKU1 (isolate N1) (HCoV-HKU1) Spike/S1 Protein (S1 Subunit, His Tag)

Human coronavirus HKU1 (isolate N1) (HCoV-HKU1) Spike/S1 Protein (S1 Subunit, His Tag)

ArtNr	40021-V08H-1
Hersteller	Sino Biological
Menge	1 mg
Quantity options	1 mg 100 ug 2 mg 20 ug 4 mg 5 ug 50 ug
Kategorie	Neuroscience Aging & Neurological Disorders Infectious Diseases Viral Infections Coronavirus (COVID-19) Peptides & Proteins Recombinant Proteins
Typ	Proteins
Specific against	other
Host	Human
Konjugat/Tag	HIS
Purity	> 90 % as determined by SDS-PAGE
Sequence	Met1-Arg760
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	S
Similar products	Spike
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Manufacturer - Type	Protein
Manufacturer - Conjugate / Tag	C-His
Shipping Temperature	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Storage Conditions	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Antigen molecule name	Spike
Protein Construction	A DNA sequence encoding the N-terminal fragment of human coronavirus (isolate HKU1) spike glycoprotein (YP_173238.1) (Met 1-Arg 760), corresponding to the S1 region, was fused with a polyhistidine tag at the C-terminus.
Expressed Host	Human Cells
Bio-Activity	Measured by its binding ability in a functional ELISA. Immobilized Spike-His (aa 1-760) (Cat: 40021-V08H) at 10 ug/ml (100 ul/well) can bind biotinylated human ACE2-Fc (Cat: 10108-H02H) with a linear range of 0.16-4.0 ug/ml.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Gly 16
Molecule Mass	The secreted recombinant human coronavirus spike glycoprotein (aa 1-760) comprises 756 amino acids with a predicted molecular mass of 85.8 kDa. As a result of high glycosylation, the apparent molecular mass of the recombinant protein is approximately 130-140 kDa in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Arbeitsanleitung (Data Sheet) anfordern

40021-V08H-1-datasheet.pdf

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Menge: 1 mg

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