Anti-Phospho-AKT (Thr342) antibody (KLH)

Store at-20°C, and avoid repeated freeze-thaw cycles.

The serine/threonine kinase Akt also known as protein kinase B (PKB) or Rac, plays a crucial role in controlling many diverse and important cellular functions such as cell survival and glycogen metabolism (Hajduch et al., 2001 and Nicholson & Anderson, 2002). Three isoforms (alpha, beta, and gamma) have been identified that can be activated rapidly in response to insulin and growth factors in a phosphoinositide 3-kinase (PI3K)-dependent fashion (Hajduch et al., 2003). Phosphorylation of Aktalpha occurs at two specific regulatory sites in Drosophila, one localized 342 in the kinase domain, Thr, and the other in the C-terminal 505 regulatory domain, Ser these two activation sites are 473 308 homologous to mammalian Ser and Thr respectively (Powell et al., 2004).

This antibody was antigen affinity purified from pooled serum.

Polyclonal

For Research Use Only (RUO).