Melanoma Cell Adhesion Molecule (MCAM) BioAssay™ ELISA Kit (Human)

Blue Ice

38220000

Melanoma Cell Adhesion Molecule (MCAM) BioAssay™ ELISA Kit (Human) is a sandwich enzyme immunoassay for the in vitro quantitative measurement of human MCAM in serum, plasma and other biological fluids.

Detection Range:
31.2-2000ng/ml

Sensitivity:
1.44ng/ml

Intra-Assay:
CV<10%

Inter-Assay:
CV<12%

Kit Components:
*026770A: Microtiter Strip, 1x96 wells, Pre-coated, ready to use
*026770B: Standard, 2x1vial 
026770C: Standard Diluent, 1x20ml
*026770D: Detection Reagent A, 1x120ul 
*026770E: Detection Reagent B, 1x120ul 
026770F: Assay Diluent A, 1x12ml
026770G: Assay Diluent B, 1x12ml
026770H: TMB Substrate, 1x9ml
026770K: Stop Solution, 1x6ml
026770L: Wash Buffer, 30X, 1x20ml

Storage and Stability:
Store *026770A, *026770B, *026770D and *026770E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Summary:
1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.
6. Aspirate and wash 5 times.
7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37°C.
8. Add 50ul Stop Solution. Read at 450nm immediately.