CCL8/MCP-2 BioAssay™ ELISA Kit, Human

Kits and Assays / Kits-ELISA (Chemokines and Chemokine Receptors), BioAssay™

4°C/-20°C

Sandwich ELISA quantitative detection kit for Human CCL8/MCP-2 in cell culture supernates. serum and plasma (heparin, EDTA).

Chemokine (C-C motif) ligand 8, also known as monocyte chemoattractant protein 2 (MCP-2), is a protein that in humans is encoded by the CCL8 gene.1 CCL8 is a small cytokine belonging to the CC chemokine family. The CCL8 protein is produced as a precursor containing 109 amino acids, which is cleaved to produce mature CCL8 containing 75 amino acids. The gene for CCL8 is encoded by 3 exons and is located within a large cluster of CC chemokines on chromosome 17q11.2 in humans.2 Monocyte chemotactic protein 2 (MCP-2) is a CC chemokine that utilizes multiple cellular receptors to attract and activate human leukocytes.

Assay Range:
15.6pg/ml-1000pg/ml

Sensitivity:
< 1 pg/ml

Crossreactivity:
There is no detectable cross-reactivity with other relevant proteins.

Uniprot ID:
P80075

Entrez Gene ID:
6355

Kit Components:
96-well plate pre-coated with anti-CCL8/MCP-2, Human
Lyophilized recombinant CCL8/MCP-2, Human standard, 10ng/tube, 2Ea
USB Cat No Kit Component
143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)
143697 Sample diluent buffer, 30ml
143698 Antibody diluent buffer, 12ml
143699 ABC diluent buffer, 12ml
143700 TMB color developing agent, 10ml
143701 TMB stop solution, 10ml

Storage and Handling:
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.