IL-12 p40 BioAssay™ ELISA Kit (Human) (Interleukin-12 subunit beta, IL-12B, Cytotoxic lymphocyte maturation factor 40kD subunit, CLMF p40, IL-12 subunit p40, NK cell stimulatory factor chain 2, NKSF2, IL12B, NKSF2)

Kits and Assays / Kits-ELISA, BioAssay™

4°C/-20°C

Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40kD (p40) subunit and a 35kD (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.

Sample Type:
Cell culture supernatants, serum, plasma, and tissue

Intended Use:
The Human IL-12 p40 BioAssay™ ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of Human IL-12 p40 concentrations in cell culture supernatants, serum, plasma, and tissue.

Sensitivity:
30pg/ml

Range:
62.5-4000pg/ml

Specificity:
Recognizes human IL-12 p40.

Sample Volume:
100ul/well

Test Principle:
This assay employs the Sandwich immunoassay technique. An anti-h IL-12 p40 monoclonal coating antibody is adsorbed onto microwells. IL-12 p40 present in the sample or standard binds to antibodies adsorbed to the microwells. Following incubation unbound sample or standard are removed during a wash step. a Biotinylated anti-h IL-12 p40 antibody is added and binds to IL-12 p40 captured by the first antibody. Following incubation unbound Biotinylated anti-h IL-12 p40 antibody is removed during a wash step. A Streptavidin-HRP is added and binds to Biotinylated anti-h IL-12 p40 antibody. Following incubation unbound Streptavidin-HRP is removed during a wash step. A colored product is formed in proportion to the amount of IL-12 p40 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-12 p40 standard dilutions and IL-12 p40 sample concentration determined.

Kit Components:
Aluminum pouches with a Microwell Plate: 8x6
Standard: 1xVial
Biotinylated antibody: 1xVial
Streptavidin-HRP: 1xVial
Assay Buffer (25ml/Vial): 1xVial
Wash Buffer Concentrate 20x (30ml/Vial): 1xVial
TMB Substrate Solution: 1x6ml
Stop Solution (12ml/Vial): 1xVial
Plate Covers- Adhesive strips: 2xstrips
Instruction: 1x

Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.