PAICS, Recombinant, Human, aa1-425, His-Tag (Phosphoribosylaminoimidazole Carboxylase, Multifunctional Protein ADE2, ADE2, ADE2H1, AIRC, PAIS, Phosphoribosylaminoimidazole-succinocarboxamide Synthase)

Blue Ice

49, 5

Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.1M sodium chloride, 1mM DTT, 20% glycerol.

PAICS, also known as phosphoribosylaminoimidazole carboxylase, is an enzyme involved in nucleotide biosynthesis and in particular in purine biosynthesis. It catalyzes the conversion of 5'-phosphoribosyl-5-aminoimidazole(AIR) into 5'-phosphoribosyl-4-carboxy-5-aminoimidazole (CAIR) as described in the reaction.

Source:
Recombinant protein corresponding to aa1-425 from human PAICS, fused to His-tag at N-terminus, expressed in E.coli.

Molecular Weight:
~49.5kD (448aa), confirmed by MALDI-TOF

Applications:
Suitable for use in SDS-PAGE. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.