Canavalia ensiformis (Concanavalin A, Jackbean)

Molecular Biology / MB-Lectin Canavalia ensiformis

-20°C

Supplied as a lyophilized powder in 0.05M Tris, pH 7.0, 0.15M sodium chloride, 0.004M calcium chloride.

Pure Canavalia ensiformis lectin (Con A) from Jackbean.

Lectins are derived from the extracts of plants, animals, viruses and microorganisms and are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms.

Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose.

Carbohydrate Specificity: alpha-D-Mannose, alpha-D-Glucose, Branched mannose.

Inhibitory Carbohydrate: Methyl alpha-D-Mannopyranoside >> alpha-D-Mannose>> alpha-D-Glucose.

Activity: Con A is a relatively weak blood agglutinin. More than 10ug/ml may be required to give visible agglutination of neuraminidase treated human erythrocytes.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.