Erythrina cristagalli (ECA) (HRP)

Molecular Biology / MB-Lectin Erythrina cristagalli

-20°C

Supplied as a liquid in 0.01M phosphate, 0.15M sodium chloride, pH 7.3. Labeled with Horseradish Peroxidase (HRP).

Pure Erythrina cristagalli lectin (ECA) from coral tree. HRP conjugated.

Lectins are derived from the extracts of plants, animals, viruses and microorganisms and are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms.

Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose.

Carbohydrate Specificity: Gal beta(1, 4)GlcNAc

Inhibitory Carbohydrate: N-acetyllactosamine > lactose > N-acetylgalactosamine > galactose

Activity:
1-2ug/ml will agglutinate neuraminidase treated human erythrocytes.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.