Glucagon-like Peptide 2 Receptor, Human, Control Peptide (GLP2R)

Molecular Biology / MB-Receptors

-20°C

Supplied as a lyophilized powder from PBS, 0.05% sodium azide.

Glucagon is a member of a multigene family comprising of Secretin, Vasoactive Intestinal Peptide (VIP), Gastric Inhibitory Peptide (GIP) and others like Glicentin and Oxyntomodulin (OXM), which differs from glucagon by C-terminal octapeptide. The glucagon precursor contains at least 3 intervening sequences that divide the protein-coding portion into 4 regions corresponding to the signal peptide and part of the N-terminal peptide, the remainder of the N-terminal peptide and glucagon, glucagon-like peptide-1 (GLP1), and GLP2. The GLP 1 & 2 stimulates intestinal growth and up regulates villus height in the small intestine, concomitant with increased crupt cell proliferation and decreased enterocyte apoptosis. The two GLP's are mainly produced in the A cells of the Islets of Langerhans in response to a drop in blood sugar concentration.
GLP2, also a processed active peptide with 33aa, (chr 2q36-q37). GLP2 regulates gastric motility, gastric acid secretion, intestinal hexose transport, and increases the barrier function of the gut epithelium. It significantly enhances the surface area of the mucosal epithelium via stimulation of crypt cell proliferation. The actions of GLP2 are transduced by the GLP2 receptor, a G-protein-coupled receptor, activation of receptor signaling in heterologous cells promotes resistance to apoptotic injury in vitro, as such it may potentially be useful in treatment of injury and dysfunction of intestinal mucosal epithelium.
GLP2 Receptor, a 550aa protein in rat and 553aa in human (chr 17p13.3). Functions as a receptor for GLP2, the activity is mediated by G proteins which activates the adenylyl cyclase, GLP2 Receptor is expressed in gut and closely related to the receptor for GLP1 (GLP1R).

Source:
20aa peptide of human GLP2R

Location:
Mid-region, Cytoplasmic

Species Sequence Homology:
Mouse-85%; Rat-75%.

Applications:
ELISA: Control peptide can be used to coat ELISA plates at 1ug/ml and detected with antibodies (See G2040-39G).

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.