SQSTM1 Antibody

SQSTM1 Antibody is a Rabbit Polyclonal antibody against SQSTM1. Sequestosome 1 (SQSTM1, p62) is a ubiquitin binding protein involved in cell signaling, oxidative stress, and autophagy (1-4). It was first identified as a protein that binds to the SH2 domain of p56Lck (5), and independently found to interact with PKCzeta (6, 7). SQSTM1 was subsequently found to interact with ubiquitin, providing a scaffold for several signaling proteins and triggering degradation of proteins through the proteasome or lysosome (8). Interaction between SQSTM1 and TRAF6 leads to the K63-linked polyubiquitination of TRAF6 and subsequent activation of the NF-kappaB pathway (9). Protein aggregates formed by SQSTM1 can be degraded by the autophagosome (4, 10, 11). SQSTM1 binds autophagosomal membrane protein LC3/Atg8, bringing SQSTM1-containing protein aggregates to the autophagosome (12). Lysosomal degradation of autophagosomes leads to a decrease in SQSTM1 levels during autophagy; conversely, autophagy inhibitors stabilize SQSTM1 levels. Studies have demonstrated a link between SQSTM1 and oxidative stress. SQSTM1 interacts with KEAP1, which is a cytoplasmic inhibitor of NRF2, a key transcription factor involved in cellular responses to oxidative stress (3). Thus, accumulation of SQSTM1 can lead to an increase in NRF2 activity.

Image Legend: Fig. 1. Western blot analysis of extracts of various cell lines, using SQSTM1 antibody. Fig. 2. Immunohistochemical analysis of paraffin-embedded Mouse kidney, using SQSTM1 antibody. Fig. 3. Immunohistochemistry analysis of paraffin-embedded rat testis, using SQSTM1 antibody. Fig. 4. Immunoprecipitation of extracts of HeLa cells incubated with SQSTM1 antibody.

Unconjugated

Affinity purified.

Q13501

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

This product is for research use only.